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DNA repair capacity has been considered to be important for the resistance of colorectal cancer cells to radiation and chemotherapy. The expression level of DNA repair genes was shown to be related to the sensitivity of carcinoma cells to treatment. We measured the level of expression of DNA repair genes in 36 sporadic colorectal adenocarcinomas, 15 adenomas, and 10 samples of normal colonic mucosa, by quantitative reverse transcription-polymerase chain reaction. The mRNA expression of p53, O6-methylguanine-DNA methyltransferase, and xeroderma pigmentosum group C, but not xeroderma pigmentosum group D, was enhanced in colorectal adenocarcinomas compared to adenomas or normal mucosa. We have analyzed these 3 genes separately in tumors with an active K-ras mutation. Expression of p53 and O6-methylguanine-DNA methyltransferase was increased in K-ras mutated tumors in comparison to tumors with a wild-type K-ras. Patients with an activated K-ras showed shorter survival time compared to patients with a wild-type K-ras. Expression of p53 and O6-methylguanine-DNA methyltransferase in tumors with a wild-type K-ras predicted poor survival. Further, we found a strong correlation between expression of p53 and O6-methylguanine-DNA methyltransferase. Patients with a tumor with an activated K-ras and a low level of expression of both DNA repair genes
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