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The common condition caused by V. parahaemolyticus is gastroenteritis, usually associated with consuming raw or undercooked shellfish harvested from Gulf and other coastal waters. The majority of cases are not illness-producing and most individuals will not seek medical care. This polyvalent organism is usually recovered from feces and no culture tests should be necessary to identify the cause of illness. Certain non-bile-salt-containing food and water additives can inhibit the growth of V. parahaemolyticus, so that tests that are designed to isolate this species must be conducted in the presence of such additives (24). Presence of Vibrio species in shellfish can be confirmed by detecting them by using diagnostic kits. Some of these kits are available commercially (24,59,93). V. cholerae O1 will grow on several nonselective agars, including thiosulfate-citrate-bile salts medium. This species may be serotyped by the agglutination technique of Kado and Loffler (62). Isolation of V. parahaemolyticus can be confirmed by means of the rapid test kit API 20E. Isolation of V. vulnificus requires several different media, including mCPC and CC agars, and observation of the organism for growth of halos in the presence of tellurite. The Gram-stain and API 20E kits alone cannot distinguish most clinically relevant species of Vibrio (24,59,62)

A rapid and inexpensive method for the identification of Vibrio sp. is provided in the Selective Medium Technique (SMT) (3). The SMT is based on a method developed for pathogenic Vibrio spp. and V. cholera and is shown in Table 2. The SMT uses charcoal and agar-based media that permit cells to form colonies with clearly visible rim zones (circles or lines) on the agar surface (1). The SMT is able to identify vibrios to the species level, including those which are motile or form colored colonies. This approach may prove useful for studies of culturable bacteria in the environment or for identifying and identifying Vibrio sp. in human fecal specimens.

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